Bacterial expression systems

Bacilli are known for their ability to secrete large amounts of biotechnologically relevant substances, which is why representatives such as Bacillus subtilis, Bacillus licheniformis or Bacillus velezensis have become crucial for the industrial production of vitamins, enzymes or secondary metabolites. In close collaboration with our academic and industrial partners, we analyze the physiology of different Bacilli and modify these cell factories. We use a variety of methods to gain insight into the growth and production behavior, expression and regulation of selected genes, or to visualize specific differentiation processes of individual cells. State-of-the-art molecular techniques for cloning and genome editing (e.g. CRISPR/Cas or Cre/lox) also allow us to create new strain variants and optimize existing Bacillus expression platforms.